The Inhibitory Effect of Sulforaphane on Bladder Cancer Cell Depends on GSH Depletion-Induced by Nrf2 Translocation

Sulforaphane (SFN), an isothiocyanate (ITCs) derived from glucosinolate that is found in cruciferous vegetables, has been reported to exert a promising anticancer effect substantial amount of scientific research. However, epidemical studies showed inconsistencies between vegetable intake and bladder cancer risk. In this study, human T24 cells were used as vitro model for revealing the inhibitory its potential mechanism SFN on cell growth. Here, low dose (2.5 µM) was shown promote proliferation (5.18–11.84%) migration cells, whilst high doses (>10 inhibited growth significantly. The induction nuclear factor (erythroid-derived 2)-like 2 (Nrf2) expression at both (10 characterized by bell-shaped curve. Nrf2 glutathione (GSH) might be underlying since siRNA GSH-depleting agent L-Buthionine-sulfoximine abolished proliferation. summary, highly dependent Nrf2-mediated GSH depletion following production. These findings suggested higher required prevention treatment cancer.